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Hardy Diagnostics Bile Spot Reagent is recommended for use in presumptively differentiating bile soluble Streptococcus pneumoniae from bile insoluble alpha-hemolytic Streptococcus spp
Sodium desoxycholate 10%
use to identify S. pneumoniae
colonies will autolyze in the presence of bile salts
The bile solubility test may be performed using a cell suspension or by applying the Bile Spot Reagent directly to the colony