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Based on naphthol AS-MX phosphate and fast blue RR salt (with citrate)
Peripheral blood or bone marrow preparations are fixed to a microscope slide
The film is then incubated in a mixture of naphthol AS-MX phosphate alkaline solution with fast blue RR salt; the resulting blue
insoluble
granular dye deposit indicates sites of alkaline phosphatase activity
Sigma-Aldrich Alkaline Phosphatase kits are intended for the histochemical semi-quantitative demonstration of alkaline phosphatase activity in leukocytes
Includes: FAST BLUE RR SALT
Catalog No. FBS25-10 CAP
FAST VIOLET B SALT
Catalog No. 851-10 CAP
NAPHTHOL AS-MX PHOSPHATE ALKALINE SOLUTION
Catalog No. 855-20 ml
MAYER’S HEMATOXYLIN SOLUTION
Catalog No. MHS1-100 ml
CITRATE CONCENTRATED SOLUTION
Catalog No. 854C-20 mL
For Semi-quantitative Demonstration of Alkaline Phosphatase Activity in White Blood Cells
