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Manufacturer: Thermo Scientific™ 78243
Thermo Scientific B-PER Reagent (in Phosphate Buffer) is an easy-to-use cell lysis reagent is a nonionic detergent-based solutions that effectively disrupt cells and solubilize native or recombinant proteins without denaturation
All B-PER Reagents are compatible with downstream applications
such as affinity chromatography (e.g.
immobilized metal affinity chromatography
glutathione chromatography)
SDS-PAGE
and protein quantification (e.g.
Pierce BCA Protein Assay
Pierce 660nm Protein Assay)
Detergent in sodium phosphate buffer; no enzyme components
Amine-free formulation for direct compatibility of lysate with amine-reactive labeling and crosslinking
Ready to use - one-step cell lysis of gram-positive and gram-negative bacteria using a mild
nonionic detergent (proprietary) in Tris or phosphate buffer formulations
B-PER Complete Reagent contains lysozyme and a universal nuclease in a single formulation with 4°C storage