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Gibco® HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media
Gibco® HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media
HEPES is a good buffering choice for many cell culture systems because it is membrane impermeable
has limited effect on biochemical reactions
is chemically and enzymatically stable
and has very low visible and UV light absorbance
The addition of 10 - 25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO2 incubator
SOLUTION, BUFFER HEPES F/CELL CULTURE MEDIA (20/CS)