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Hardy Diagnostics HardyDisks™ Nitrate are for the differentiation of anaerobes based on the ability to reduce nitrate
The HardyDisk™ Nitrate detects the nitrite that organisms produce by the colored end product that is formed after addition of nitrate reagents
Nitrate
when combined with sulfanilic acid and N
N-dimethyl-alpha-naphthylamine forms a red compound
p-sulfobenzene-azo-napthylamine
which is indicative of a positive reaction
If there is no color production (a negative reaction) zinc dust is added to confirm the negative reaction
HardyDisks™ Nitrate are impregnated with potassium nitrate and sodium molybdate
